Skip to content

xa2multi.pl issue creating secondary alignments from hard-clipped primary #446

@sjaenick

Description

@sjaenick

Hi @lh3,

the attached SAM entry (read aligned to hg38)

bug_sam.txt

is hard-clipped (79H70M), i.e. only the matching 70 nucleotides are found in the SEQ field. Passing this to xa2multi.pl,
the next alignment (79S70M) is generated from the XA tag. Here, SEQ should include the soft-clipped bases, but they
are missing (and cannot be obtained from the initial entry).

Only reasonable fix would be to make xa2multi.pl obtain SEQ from the original FASTQ file, I guess.

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions