Skip to content

Reads with mapq=0 not mapped to the same location as their mates #405

@pinbo

Description

@pinbo

Hi Developers, I am working on wheat, which is allohexaploid with 3 highly similar subgenomes (A, B, D). Genes across the 3 genomes share about 97% similarity, so for paired end reads, sometimes one read could be equally mapped to all 3 subgenomes (say chromosome 1A and 1B and 1D, mapq=0), but its mate is uniquely mapped to one subgenome (say 1A) due to 1 to a few SNPs. I think for this situation, bwa mem should put the read on the same chromosome (1A) as its mate, but actually bwa mem seems to randomly select a primary location without considering its mate's location. I checked minimap2 and bowtie2, and they all put the reads on the same chromosome for this situation (one read has mapq0 and its mate has mapq>0). Could you fix this?

Thanks,

Junli Zhang

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions